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Semidry Electroblotting of Peptides and Proteins from Acid–Urea Polyacrylamide Gels

✍ Scribed by Min-Shine Chow Wang; Jason S. Pang; Michael E. Selsted

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
943 KB
Volume
253
Category
Article
ISSN
0003-2697

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✦ Synopsis

filter paper reservoirs, obviating the need for tank im-A semidry electrophoretic transfer method was demersion, and transfer times were reduced to less than veloped for efficient electroblotting of proteins sepa-1 h. Due to its convenience and speed, semidry transfer rated by acid-urea polyacrylamide gel electrophoresis has been adopted to the electroblotting of DNA and (AU-PAGE). Model polypeptides ranging from 1.8 to RNA (13, 14).

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