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Extraction of proteins and peptides from Coomassie blue-stained sodium dodecyl sulfate-polyacrylamide gels

✍ Scribed by D.R. Gibson; R.W. Gracy

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 212 KB
Volume: 96
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90592-x

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✦ Synopsis

A method is described for extracting proteins and peptides from stained sodium dodecyl sulfate-polyacrylamide gels. Coomassie blue and sodium dodecyl sulfate present in stained gel sections are removed to allow subsequent analysis of the peptides (e.g., amino acid analysis or tryptic digestion and fingerprinting). The method is simple, requires no radioisotopes or special equipment, and can be carried out with a minimum of handling of the sample. The process can be used for samples at the nanomole level with recoveries of 90%.

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