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Fast Staining and Destaining of Sodium Dodecyl Sulfate–Polyacrylamide Gels

✍ Scribed by Matthew D. Lloyd

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
36 KB
Volume
241
Category
Article
ISSN
0003-2697

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✍ H.J. Moller; J.H. Poulsen 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 281 KB

A method for detection of glycoproteins in thin sodium dodecyl sulfate polyacrylamide gels was developed by a combination of (i) initial periodic acid oxidation/Alcian blue staining and (ii) subsequent staining with silver nitrate. The procedure allowed detection of as little as \(1.6 \mathrm{ng}\)