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Activity staining of pectinesterase on polyacrylamide gels after acidic or sodium dodecyl sulfate electrophoresis

โœ Scribed by Wen-Chi Hou; Dr. Yaw-Huei Lin


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
359 KB
Volume
19
Category
Article
ISSN
0173-0835

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โœ J. Venegas; A. Solari ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 442 KB

A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- or biotin-labeled deoxynucleotides during DNA synthesis, and their detecti