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The Detection of Enzyme Activity Following Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis

✍ Scribed by Kenneth M. Bischoff; Liang Shi; Peter J. Kennelly

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
266 KB
Volume
260
Category
Article
ISSN
0003-2697

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Colorimetric Detection of DNA Polymerase
Colorimetric Detection of DNA Polymerase Activity after Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
✍ J. Venegas; A. Solari 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 442 KB

A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- or biotin-labeled deoxynucleotides during DNA synthesis, and their detecti