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Improved detection of amylase activity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with copolymerized starch

✍ Scribed by Tomás F. Martínez; Francisco J. Alarcón; Manuel Díaz-López; Francisco J. Moyano


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
76 KB
Volume
21
Category
Article
ISSN
0173-0835

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A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- or biotin-labeled deoxynucleotides during DNA synthesis, and their detecti