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Detection of Calcium Binding Proteins by Two-Dimensional Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis

✍ Scribed by Isamu Kameshita; Hitoshi Fujisawa


Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
316 KB
Volume
249
Category
Article
ISSN
0003-2697

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Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip