An improved procedure, "thermoblotting", is described for transferring proteins by diffusion from PhastGel Gradient media to an immobilizing matrix after horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After electrophoresis the gels were left on the separation bed of PhastSyste
✦ LIBER ✦
Centrifuge-blotting of proteins after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis
✍ Scribed by Leonila F. Hermansen; Øyvind Pedersen; Prof. Knut Sletten
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 431 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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## Abstract As a follow‐up of our previous report (__Anal. Chem.__ 2007, __79__, 821–827) on analytical SDS‐PAGE focusing, a refinement of the method for separation of peptides in the small to medium __M__~r~ range (0.5–10 kDa) is here reported, based on a shallow gradient of immobilized positive c