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Detection of gelatinolytic enzyme activities after sodium dodecyl sulfate-electrophoresis and protein blotting

✍ Scribed by Jiri Moos


Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
221 KB
Volume
12
Category
Article
ISSN
0173-0835

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Blotting from PhastGel media after horiz
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An improved procedure, "thermoblotting", is described for transferring proteins by diffusion from PhastGel Gradient media to an immobilizing matrix after horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After electrophoresis the gels were left on the separation bed of PhastSyste