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Recovery of functional proteins from sodium dodecyl sulfate-polyacrylamide gels

โœ Scribed by Fumio Hanaoka; Joseph L. Shaw; Gerald C. Mueller

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
424 KB
Volume
99
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple method and apparatus are described for the efficient recovery of proteins from sodium dodecyl sulfate-polyacrylamide gel systems after electrophoretic resolution. This procedure provides for high yields of proteins which are free of sodium dodecyl sulfate and in certain cases, exhibit significant levels of biological activity.

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Extraction of proteins and peptides from Coomassie blue-stained sodium dodecyl sulfate-polyacrylamide gels
โœ D.R. Gibson; R.W. Gracy ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 212 KB

A method is described for extracting proteins and peptides from stained sodium dodecyl sulfate-polyacrylamide gels. Coomassie blue and sodium dodecyl sulfate present in stained gel sections are removed to allow subsequent analysis of the peptides (e.g., amino acid analysis or tryptic digestion and f