A method for recovery of proteins from sodium dodecyl sulfate-polyacrylamide gels
β Scribed by Lalio Djondjurov; Howard Holtzer
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 446 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A simple method and apparatus are described for the efficient recovery of proteins from sodium dodecyl sulfate-polyacrylamide gel systems after electrophoretic resolution. This procedure provides for high yields of proteins which are free of sodium dodecyl sulfate and in certain cases, exhibit signi
dling and storage. The overall quality ofthe second-dimension gel is improved by eliminating the dark carrier ampholyte streak at the front of the basic end of the gel as well as the SDS-Triton artifact at the acidic end. The degree of protein resolution is essentially identical for the two techniqu