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Quantitation of submicrogram amounts of protein using Coomassie brilliant blue R on sodium dodecyl sulfate-polyacrylamide slab-gels

✍ Scribed by Richard Kahn; Robert W. Rubin

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 272 KB
Volume: 67
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90305-x

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✦ Synopsis

Quantitation of Submicrogram Amounts of Protein

Using Coomassie Brilliant Blue R on Sodium Dodecyl Sulfate-Polyacrylamide Slab-Gels A sodium dodecyl sulfate (SDS)-polyacrylamide slab-gel system was used to study the use of Coomassie brilliant blue (CB) as a quantitative stain. Quantitation curves are shown for tubulin, cytochrome c, histone, and actin from gels stained with CB. A comparison was made of three identical gels using an actin sample and stained with CB, fast green and buffalo black. CB staining was found to be quantitative in the 0.05-2.75pg range as well as possessing an order of magnitude increase in sensitivity over other stains tested.

Methods

Gels. The polyacrylamide thin (16 X 12 X 0.08 cm) slab-gel system of Laemmli (6) and O'Farrell et al. ( 7) was used, giving gels with an acrylamide concentration of 12% and a sodium dodecyl sulfate (SDS) 347

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