Pt. A (v. 78) -- Pt. B (v. 79) -- Pt. C (v. 119). Edited By Sidney Pestka. Includes Bibliographical References And Indexes.
Amino acid analysis with fluorescamine of stained protein bands from polyacrylamide gels
✍ Scribed by S. Stein; C.H. Chang; P. Böhlen; K. Imai; S. Udenfriend
- Book ID
- 107711551
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 351 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
A single stained band containing approximately 5 fig of protein was cut out of a polyacrylamide gel and subjected to hydrolysis together with the gel. The hydrolysate was subsequently analyzed for its amino acid content by high-performance liquid chromatography and postlabeling with o-phthalaldehyde
A procedure which allows quantitative determination of the amino acid composition of stained proteins separated by sodium dodecyl sulfate (SDS)-gel electrophoresis using ninhydrin is described. The Coomassie-protein-SDS complex extracted from the gel is dialyzed, lyophylized, and subjected to amino
It is shown that the contribution of the gel itself to amino acid compositions of proteins eluted from polyacrylamide gels is significant (especially at low protein-to-gel ratios), linearly related to the volume of the gel slices eluted, and, when applied as a correction to amino acid composition da