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Amino acid analysis by high-performance liquid chromatography of a single stained protein band from a polyacrylamide gel

โœ Scribed by Yoko Hashimoto; Sadako Yamagata; Taro Hayakawa

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
591 KB
Volume
160
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A single stained band containing approximately 5 fig of protein was cut out of a polyacrylamide gel and subjected to hydrolysis together with the gel. The hydrolysate was subsequently analyzed for its amino acid content by high-performance liquid chromatography and postlabeling with o-phthalaldehyde. Bovine serum albumin, ribonuclease B, ovalbumin, pepsin, and chymotrypsinogen A were analyzed by this method, and their amino acid compositions were found to be in good agreement with the reported values.

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