A simple, specific radiometric assay for 5′-nucleotidase
✍ Scribed by Sunil K. Chatterjee; Malaya Bhattacharya; Joseph J. Barlow
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 700 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A sensitive assay for 5'-nucleotidase activity is described. The method involves incubation with radiolabeled [3H]adenosine 5'-monophosphate (AMP) and thinlayer chromatography on polyethyleneimine-coated plastic to achieve separation of substrate (AMP) from product (adenosine). The resulting spots c
Male Wistar rats weighing 200-250 g were used. I-d,-Estrtrtliol. To a solution of 2-iodoestradiol (16) (IO mg) and PdCl, (10 mg) dissolved in methanol (0.7 ml)-D,O (0.1 ml) was added NaBD, (2 mg), and the resulting solution was stirred at 0°C for 40 min under a stream of nitrogen gas. The reaction