A simple radiometric in vitro assay for acetylcholinesterase inhibitors
β Scribed by Tomas R. Guilarte; H. Donald Burns; Robert F. Dannals; Henry N. Wagner Jr.
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 342 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0022-3549
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π SIMILAR VOLUMES
A rapid and simple radiometric assay for cholinesterase, suitable for multiple determinations, has been developed. (aH-acetyl) choline is enzymatically hydrolyzed in a small reaction volume in a scintillation vial. The released [all]acetate is then extracted into a toluene-based scintillator added d
A gas chromatographichass spectrometric assay for quantifying two catechol estrogens, 2-hydroxyestradiol and 4-hydroxyestradiol, in microsomal preparations is described. The assay employs deuterium-labeled analogs of the catechol estrogens as internal standards and permits quantification of catechol
A procedure for the qualitative assessment of inhibitory activity towards acetylcholinesterase for a given compound is described. Solutions of the compounds of interest are spotted on silica gel TLC plates in a matrix pattern. The silica gel plate is sprayed with a solution of acetylthiocholine iodi