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A rapid, simple radiometric assay for cholinesterase, suitable for multiple determinations

✍ Scribed by Carl D. Johnson; Richard L. Russell

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
471 KB
Volume
64
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid and simple radiometric assay for cholinesterase, suitable for multiple determinations, has been developed. (aH-acetyl) choline is enzymatically hydrolyzed in a small reaction volume in a scintillation vial. The released [all]acetate is then extracted into a toluene-based scintillator added directly to the vial, without removing the reaction volume. The extracted [3H]acetate counts efficiently, but the unhydrolyzed [3H]acetylcholine remains unextracted in the small aqueous reaction volume, from which its weak fl-particles of decay do not escape to excite the scintillator. The assay is highly reproducible, quite sensitive, and useful for applications in which multiple samples must be quickly assayed.

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