A simple and rapid method for isolating cassava leaf linamarase suitable for cassava cyanide determination

✍ Scribed by Yeoh, Hock-Hin; Bradbury, J Howard; Egan, Sylvia V

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 169 KB
• Volume: 75
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(199710)75:2<258::aid-jsfa873>3.0.co;2-m

✦ Synopsis

A simple method was developed for isolating cassava leaf linamarase. It involved homogenising the leaves in a bu †er containing polyvinylpolypyrrolidone, followed by ammonium sulphate precipitation, Ðltration and partial puriÐcation of the enzyme by hydrophobic interaction chromatography. All stages of the enzyme preparation were carried out at room temperature and it was completed within 90 min. A linamarin-indicator strip for checking the activity of the enzyme preparation was also developed. This isolation technique which has been developed into a simple kit should be suitable for laboratories in developing countries where the enzyme is needed to determine the cyanogenic potential of cassava and its products.