✦ LIBER ✦

A rapid radiometric assay for dihydrofolate reductase

✍ Scribed by Ronald Hayman; Roland McGready; Martin B. Van der Weyden

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 381 KB
Volume: 87
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90696-6

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid radiochemical procedure for the measurement of dihydrofolate reductase activity is described. The method employs separation of the radiolabeled substrate from the products ofthe reaction by precipitation with acetic acid and zinc sulfate. This method permits rapid processing of samples and eliminates time-consuming column chromatography techniques. Good agreement of results is obtained when the radioactive method is compared to the spectrophotometric assay method.

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