A rapid radiochemical procedure for the measurement of dihydrofolate reductase activity is described. The method employs separation of the radiolabeled substrate from the products ofthe reaction by precipitation with acetic acid and zinc sulfate. This method permits rapid processing of samples and e
A fluorometric assay for dihydrofolate reductase
โ Scribed by C.A. Lindquist; E.C. Cadman; J.R. Bertino
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 290 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
This report describes a simple method to measure the activity of dihydrofolate reductase using the substrate [3H]dihydrofolate, which is generated by preincubation of [3H]folic acid for 10 min with dithionite before the enzymatic reaction. The procedure then measures the direct reduction of [3H]dihy
A fluorometric method for the assay of cholesterol reductase activity from pea leaves (Pisum sativum) is presented. This method is based on the decrease in relative fluorescence occurring as a result of the oxidation of NADH when cholesterol is reduced catalytically to coprostanol by cholesterol red
A microfluorometric adaptation of the method of D. E. Paglia and W. N. Valentine has been made which can assay glutathione peroxidase activity in less than 100 pg of tissue. As in the original method, the oxidized glutathione produced in the reaction is coupled to the oxidation of NADPH by glutathio
A rapid and sensitive fluorometric assay for measuring the activity of proline reductase is described. The product of the enzymic reaction, &aminovalerate, is converted to a highly fluorescent derivative by reaction with o-phthalaldehyde. The water-soluble fluorescent product exhibits an excitation
In 1959 Shore, Burkhalter and Cohn (1) reported that the reaction of o-phthalaldehyde with histamine yielded a highly fluorescent reaction product which could be used for the assay of submicrogram amounts of histamine in biological tissues. Modification of this reaction and/or the extraction techniq