✦ LIBER ✦

Proline reductase: A sensitive fluorometric assay with o-phthalaldehyde

✍ Scribed by Belinda Seto

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 244 KB
Volume: 95
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90183-0

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✦ Synopsis

A rapid and sensitive fluorometric assay for measuring the activity of proline reductase is described. The product of the enzymic reaction, &aminovalerate, is converted to a highly fluorescent derivative by reaction with o-phthalaldehyde. The water-soluble fluorescent product exhibits an excitation maximum at 340 nm and an emission maximum at 4.55 nm. The fluorophor reacts specifically with Gaminovalerate without any interference from proline.

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