A Fluorometric Assay for the Quantification of RNA in Solution with Nanogram Sensitivity

✍ Scribed by David M. Schmidt; Joel D. Ernst

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 64 KB
• Volume: 232
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.0025

✦ Synopsis

Visual detection limit Staining reagent (ng biotinylated DNA per Time for color Stability of composition slot blot) Color of stain development stain color Complete reagent 11 Yellow-brown 10-15 min 4 weeks Complete reagent without 11 Yellow-brown 10-15 min 4 weeks luminol Complete reagent without 25 Gray-green 12 h 4 weeks para-iodophenol 101 complete reagent 11 Yellow-brown Less than 1 min 24 h Millipore positively 11 Yellow-brown 10-15 min 4 weeks charged membranes Tropix negatively charged 11 Yellow-brown 10-15 min 4 weeks membranes 4. Cercek, B., Obremski, R. J., and Oh, C. S. (1990) in Bioluminesgray-green-colored precipitate. It has been suggested cence and Chemiluminescence: Current Status. Proceedings of that this insoluble product is produced by a polymerizathe VIth International Symposium on Bioluminescence and tion reaction involving up to 11 compounds of which Chemiluminescence, Cambridge (Stanley, P. E., and Kricka, only 3-aminophthalimide has been identified (5). Since