A gas chromatographic/mass spectrometric assay for catechol estrogens in microsomal incubations: Comparison with a radiometric assay

✍ Scribed by D. J. Porubek; S. D. Nelson

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 439 KB
• Volume: 15
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200150307

✦ Synopsis

A gas chromatographichass spectrometric assay for quantifying two catechol estrogens, 2-hydroxyestradiol and 4-hydroxyestradiol, in microsomal preparations is described. The assay employs deuterium-labeled analogs of the catechol estrogens as internal standards and permits quantification of catechol estrogens, in microsomal incubations, at low (1-2) p~ concentrations. The compounds are analyzed as their trimethylsilyl derivatives following separation by capillary gas chromatography.