𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A simple, sensitive radiomicroassay for 5′-nucleotidase

✍ Scribed by M.Y. Hurwitz; R.D. Edstrom

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
254 KB
Volume
84
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A sensitive assay for 5'-nucleotidase activity is described. The method involves incubation with radiolabeled [3H]adenosine 5'-monophosphate (AMP) and thinlayer chromatography on polyethyleneimine-coated plastic to achieve separation of substrate (AMP) from product (adenosine). The resulting spots can be excised and counted in a scintillation counter to give a quantitative determination of 5'-nucleotidase activity. The method allows detection of less than 1.5 x 10e5 units of enzyme.

📜 SIMILAR VOLUMES

Simple radioassay for uridine phosphoryl
Simple radioassay for uridine phosphorylase and 5′-nucleotidase
✍ Harutoshi Kizaki; George Weber 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 350 KB

A simple micromethod was developed for the accurate measurement of the activity of uridine phosphorylase in the direction of both ribosylation and phosphorolysis. The technique was applicable also to the measurement of cytoplasmic Y-nucleotidase. In this micromethod the radioactive products were sep

A simple and sensitive assay for dopamin
A simple and sensitive assay for dopamine-β-hydroxylase
✍ Tong Hyub Joh; Robert A. Ross; Donald J. Reis 📂 Article 📅 1974 🏛 Elsevier Science 🌐 English ⚖ 349 KB

A simple, rapid, and highly sensitive radiochemical assay for measuring the activity of dopamine+hydroxylase in tissues and serum is described. Enzyme activity is detected by converting [l-'"Cltyramine to [l-"Cloctopamine which is then subjected to periodate cleavage to form ["Clformamide. This radi

A simple and sensitive fluorometric assa
A simple and sensitive fluorometric assay for tyrosine hydroxylase
✍ Takashi Yamauchi; Hitoshi Fujisawa 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 419 KB

A simple, rapid, and sensitive fluorometric assay for measuring the activity of tyrosine hydroxylase is described. The enzyme activity is detected by converting tyrosine to 3,4-dihydroxyphenylalanine (dopa), which is then subjected to conversion to the highly fluorescent product by the trihydroxyind