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A simple and sensitive assay for dopamine-β-hydroxylase

✍ Scribed by Tong Hyub Joh; Robert A. Ross; Donald J. Reis

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
349 KB
Volume
62
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple, rapid, and highly sensitive radiochemical assay for measuring the activity of dopamine+hydroxylase in tissues and serum is described. Enzyme activity is detected by converting [l-'"Cltyramine to [l-"Cloctopamine which is then subjected to periodate cleavage to form ["Clformamide. This radiolabeled product is oxidized to "CO, by addition of permanganate and the "CO, is trapped and counted. The assay is simple and sensitive, it can linearly detect enzyme in all tissues with a wide range of activity, it uses maximal concentration of substrate, and it requires the addition of only one concentration of EM1 to block endogenous inhibitor(s) in different tissues or enzyme concentrations.

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