𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Simple radioassay for uridine phosphorylase and 5′-nucleotidase

✍ Scribed by Harutoshi Kizaki; George Weber

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
350 KB
Volume
105
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A simple micromethod was developed for the accurate measurement of the activity of uridine phosphorylase in the direction of both ribosylation and phosphorolysis. The technique was applicable also to the measurement of cytoplasmic Y-nucleotidase. In this micromethod the radioactive products were separated from the substrates by electrophoresis on cellulose acetate membrane in 0.1 M borate buffer, pH 9.0, at 150 V for 40 min. These micromethods are highly sensitive and capable of utilizing small samples (4-30 pg protein); they are valid in crude tissue extracts of rat liver and hepatoma.

📜 SIMILAR VOLUMES

A simple, sensitive radiomicroassay for
A simple, sensitive radiomicroassay for 5′-nucleotidase
✍ M.Y. Hurwitz; R.D. Edstrom 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 254 KB

A sensitive assay for 5'-nucleotidase activity is described. The method involves incubation with radiolabeled [3H]adenosine 5'-monophosphate (AMP) and thinlayer chromatography on polyethyleneimine-coated plastic to achieve separation of substrate (AMP) from product (adenosine). The resulting spots c

A rapid and simple radioassay for tyrosi
A rapid and simple radioassay for tyrosine hydroxylase activity
✍ Toshiharu Nagatsu; Morton Levitt; Sidney Udenfriend 📂 Article 📅 1964 🏛 Elsevier Science 🌐 English ⚖ 269 KB

## COMJIUNICATIONs muscle and kidney at pH 4.6 yields a filtrate containing all the tissue magnesium and calcium, with amounts of heavy metals too low to interfere with EDTA titration of these elements using eriochrome black T or Cal-Red as indicators.