๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A rapid and simple radioassay for tyrosine hydroxylase activity

โœ Scribed by Toshiharu Nagatsu; Morton Levitt; Sidney Udenfriend


Publisher
Elsevier Science
Year
1964
Tongue
English
Weight
269 KB
Volume
9
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis


COMJIUNICATIONs

muscle and kidney at pH 4.6 yields a filtrate containing all the tissue magnesium and calcium, with amounts of heavy metals too low to interfere with EDTA titration of these elements using eriochrome black T or Cal-Red as indicators.


๐Ÿ“œ SIMILAR VOLUMES


A rapid and simplified assay method for
โœ Robert A. Levine; Harvey B. Pollard; Donald M. Kuhn ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 335 KB

Tyrosine hydroxylase can be measured by release of tritiated water from labeled tyrosine, and the assay method has now been modified to allow recovery of 3H2O from the reaction mixture in a much more rapid and less tedious manner than previously possible. In the new method, the tyrosine hydroxylase

A simple and sensitive fluorometric assa
โœ Takashi Yamauchi; Hitoshi Fujisawa ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 419 KB

A simple, rapid, and sensitive fluorometric assay for measuring the activity of tyrosine hydroxylase is described. The enzyme activity is detected by converting tyrosine to 3,4-dihydroxyphenylalanine (dopa), which is then subjected to conversion to the highly fluorescent product by the trihydroxyind

A rapid assay for prolyl 3-hydroxylase a
โœ Juha Risteli; Karl Tryggvason; Kari I. Kivirikko ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 451 KB

An assay is reported for prolyl 3-hydroxylase activity. The method is based on the release of tritiated water (THO) during 3-hydroxylation of a 2,3-T-~proline-labeled (T = tritium) polypeptide substrate in which all prolyl residues recog nized by prolyl 4-hydroxylase have been converted to 4-hydroxy

A rapid radioassay for folic acid reduct
โœ Sheldon P. Rothenberg ๐Ÿ“‚ Article ๐Ÿ“… 1966 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 245 KB

The enzymic reduction of isotopically labeled folic acid to tetrahydrofolic acid (H,-folate) employing folic acid reductase extracted from chicken liver has been previously reported from this laboratory (1, 2). Using high specific activity tritiated folic acid (5 to 25 c/mmole) the reduction of subs