Recent studies in this laboratory (1, 2) have revealed new information about the mechanism of the phenylalanine hydroxylase reaction. Briefly, these experiments show that during hydroxylation the para substituent of the substrate, phenylalanine, migrates and appears on the meta position(s) of the pr
A simple, specific, radioisotopic assay for 5′-nucleotidase
✍ Scribed by Mary Kay Gentry; Ray A. Olsson
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 209 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0003-2697
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A sensitive assay for 5'-nucleotidase activity is described. The method involves incubation with radiolabeled [3H]adenosine 5'-monophosphate (AMP) and thinlayer chromatography on polyethyleneimine-coated plastic to achieve separation of substrate (AMP) from product (adenosine). The resulting spots c
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