A simple radiometric assay for estradiol 2-hydroxylase activity

✍ Scribed by Mitsuteru Numazawa; Yoko Kiyono; Toshio Nambara

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 606 KB
• Volume: 104
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90077-9

✦ Synopsis

Male Wistar rats weighing 200-250 g were used.

I-d,-Estrtrtliol.

To a solution of 2-iodoestradiol (16) (IO mg) and PdCl, (10 mg) dissolved in methanol (0.7 ml)-D,O (0.1 ml) was added NaBD, (2 mg), and the resulting solution was stirred at 0°C for 40 min under a stream of nitrogen gas. The reaction mixture was poured into 1% HCI and then