Male Wistar rats weighing 200-250 g were used. I-d,-Estrtrtliol. To a solution of 2-iodoestradiol (16) (IO mg) and PdCl, (10 mg) dissolved in methanol (0.7 ml)-D,O (0.1 ml) was added NaBD, (2 mg), and the resulting solution was stirred at 0ยฐC for 40 min under a stream of nitrogen gas. The reaction
A simple radiometric assay for microsomal aryl hydroxylase activity
โ Scribed by John W. Daly
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 653 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Recent studies in this laboratory (1, 2) have revealed new information about the mechanism of the phenylalanine hydroxylase reaction. Briefly, these experiments show that during hydroxylation the para substituent of the substrate, phenylalanine, migrates and appears on the meta position(s) of the pr
A simple and rapid method for the determination of (S)-mephenytoin 4-hydroxylase activity by human liver microsomal cytochrome P-450 has been developed. [Methyl-'4C]mephenytoin was synthesized by alkylation of Snirvanol with 14CHJ and used as a substrate. AAer incubation of [mefhy/-'4C]mephenytoin w