𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A rapid method for preparation of 32P-applicators

✍ Scribed by J. Svobodová; L. Kronrád; J. Kolínská

Publisher
Elsevier Science
Year
1971
Weight
82 KB
Volume
22
Category
Article
ISSN
0020-708X

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Preparation of 3′−32P-coenzyme A
Preparation of 3′−32P-coenzyme A
✍ Eberhard G. Trams; John E. Franklin; Carl J. Lauter 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 French ⚖ 138 KB

A convenient labeling technique for the nucleotide portion of CoASH consists of the introduction of 32phosphorus at the 3'-position of the ribosyl moiety of CoA. Adenine pantetheine dinucleotide was prepared according to Wang ( I ) by treatment of CoA with a 3 '-nucleotidase (3.1.3.6). Isotopic phos

A rapid boiling method for the preparati
A rapid boiling method for the preparation of bacterial plasmids
✍ David S. Holmes; Michael Quigley 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 1005 KB

A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15–40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca