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A rapid boiling method for the preparation of bacterial plasmids

✍ Scribed by David S. Holmes; Michael Quigley

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
1005 KB
Volume
114
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

A rapid boiling method for the preparati
A rapid boiling method for the preparation of bacterial plasmids
✍ David S. Holmes; Michael Quigley πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 1005 KB

A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15–40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca

A note on the use of CsCl centrifugation
A note on the use of CsCl centrifugation to purify bacterial plasmids prepared by the rapid boiling method
✍ A.J. Eisenberg; David S. Holmes πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 83 KB

A modification of the rapid boiling method for the preparation of bacterial plasmids allows it to be readily used in conjunction with standard CsCI-ethidium bromide density centrifugation for the-isolation of purified plasmids.

A method for the preparation of undigest
A method for the preparation of undigested bacterial cell walls
✍ Yoshida, A. ;HedΓ©n, C.-G. ;Cedergren, B. ;Edebo, L. πŸ“‚ Article πŸ“… 1961 πŸ› Wiley (John Wiley & Sons) βš– 554 KB

## Abstract The combination of low‐temperature disintegration with gradient and pile centrifugation makes possible the preparation of bacterial cell walls under conditions preventing digestion.