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A note on the use of CsCl centrifugation to purify bacterial plasmids prepared by the rapid boiling method

โœ Scribed by A.J. Eisenberg; David S. Holmes


Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
83 KB
Volume
127
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


A modification of the rapid boiling method for the preparation of bacterial plasmids allows it to be readily used in conjunction with standard CsCI-ethidium bromide density centrifugation for the-isolation of purified plasmids.


๐Ÿ“œ SIMILAR VOLUMES


A rapid boiling method for the preparati
โœ David S. Holmes; Michael Quigley ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 1005 KB

A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15โ€“40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca