A rapid and convenient method for preparing salt-free [γ-32P]ATP
✍ Scribed by John L. Palmer; Joseph Avruch
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 139 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a
A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P
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