A new method for the rapid analysis of inorganic pyrophosphate (PPJ which utilizes the enzyme ATP sulfurylase is described. All components of the assay system are commercially available and inexpensive. The assay is linear over the range of 0.5-50.0 nmol of PP, and is not affected by inorganic phosp
A method for rapid separation of [32P]-inorganic phosphate or pyrophosphate from ATP
โ Scribed by K.P. Huang
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 303 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P
A method is described for the separation and assay of radiolabeled glycosaminoglycuronans (GAGS) from cell culture medium. Following papain digestion and precipitation with cetylpyridinium chloride onto cellulose ester filter membrane, hyaluronic acid can be separated from sulfated GAGS by selective
A simple and rapid procedure for the synthesis of AMP"0, AMP'sO, and the corresponding guanosine derivatives is described. The method is based on the intermediate production of P"OC13 or P'\*OClj from PCIs, but does not involve their isolation, so that all steps involved can be carried out in the sa