๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A rapid method for separation and assay of radiolabeled mucopolysaccharides from cell culture medium

โœ Scribed by Heikki Saarni; Markku Tammi

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
308 KB
Volume
81
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A method is described for the separation and assay of radiolabeled glycosaminoglycuronans (GAGS) from cell culture medium. Following papain digestion and precipitation with cetylpyridinium chloride onto cellulose ester filter membrane, hyaluronic acid can be separated from sulfated GAGS by selective elution with HCI.

๐Ÿ“œ SIMILAR VOLUMES

A convenient and rapid procedure for ext
A convenient and rapid procedure for extraction of RNA polymerase II from cultured mammalian cells
โœ K.C. Gupta; M.W. Taylor ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 445 KB

A convenient and rapid procedure has been developed to extract RNA polymerase II quantitatively from cultured mammalian cells. The procedure was successfully employed on HeLa, CHO, L, and MDBK cells. The easy extraction of RNA polymerase II by freeze-thaw of cells in a buffer of low ionic strength s

A rapid method for the preparation of si
A rapid method for the preparation of single-cell suspensions from solid tissues
โœ Narendra P. Singh ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 66 KB ๐Ÿ‘ 2 views

A simple and rapid methodology for the preparation of single-cell suspension from animal tissues is described. It uses a device called a "tissue press" to mince the tissue into small pieces of desired sizes which are then dissociated into single cells using a pipette. The device is inexpensive and t

A simple, rapid, and sensitive method fo
A simple, rapid, and sensitive method for estimation of DNA, RNA, and protein synthesis in carrot cell cultures
โœ Thomas E. Ferrari; Jack M. Widholm ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 390 KB

A simple, rapid, and sensitive method for the measurement of DNA, RNA, and protein synthesis in cell samples is presented. Tke method measures the amount of isotope incorporated into these macromolecules after cell collection on filter paper and washing with a methanol:chloroform:water mixture. Samp