A rapid method for the preparation of single-cell suspensions from solid tissues

✍ Scribed by Narendra P. Singh

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 66 KB
• Volume: 31
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/(sici)1097-0320(19980301)31:3<229::aid-cyto10>3.0.co;2-t

✦ Synopsis

A simple and rapid methodology for the preparation of single-cell suspension from animal tissues is described. It uses a device called a "tissue press" to mince the tissue into small pieces of desired sizes which are then dissociated into single cells using a pipette. The device is inexpensive and the method economizes time and labor. An example of its use in DNA double-strand breaks study, using microgel electrophoresis, is described.