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A convenient and rapid procedure for extraction of RNA polymerase II from cultured mammalian cells

✍ Scribed by K.C. Gupta; M.W. Taylor

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 445 KB
Volume: 82
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90177-4

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✦ Synopsis

A convenient and rapid procedure has been developed to extract RNA polymerase II quantitatively from cultured mammalian cells. The procedure was successfully employed on HeLa, CHO, L, and MDBK cells. The easy extraction of RNA polymerase II by freeze-thaw of cells in a buffer of low ionic strength suggests that the enzyme is not tightly bound to chromatin. 3%

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