✦ LIBER ✦

A simple, rapid, and sensitive method for estimation of DNA, RNA, and protein synthesis in carrot cell cultures

✍ Scribed by Thomas E. Ferrari; Jack M. Widholm

Publisher: Elsevier Science
Year: 1973
Tongue: English
Weight: 390 KB
Volume: 56
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(73)90200-5

No coin nor oath required. For personal study only.

✦ Synopsis

A simple, rapid, and sensitive method for the measurement of DNA, RNA, and protein synthesis in cell samples is presented. Tke method measures the amount of isotope incorporated into these macromolecules after cell collection on filter paper and washing with a methanol:chloroform:water mixture. Samples as low as 50 pg in size can be prepared in less than 30 set for measurement of radioactivity.

Methods

Carrot, (Caucus carota) cultures were grown in 250-ml Erlenrneyer flasks containing, 100 ml of liquid medium on a reciprocating shaker at 346

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