✦ LIBER ✦

Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates

✍ Scribed by Uwe Karsten; Albert Wollenberger

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 368 KB
Volume: 77
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90259-7

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✦ Synopsis

In the DNA and RNA assays carried out with the use of ethidium bromide in cell and tissue homogenates according to the method of Karsten and Wollenberger (1). Pronase can be replaced to advantage by heparin in the nucleoprotein dissociation step. Furthermore, rhodamine B is used as a standard instead of DNA. Attention is called to several methodical details.

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