A simple, rapid, and sensitive method for measuring protein concentration in subcellular membrane fractions prepared by sucrose density ultracentrifugation
✍ Scribed by Ian A. Simpson; Ole Sonne
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 287 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The Multiskan spectrophotometric system and Coomassie brilliant blue G-250 protein-dye binding method have been used together to measure NaOH-solubilized protein in subcellular membrane fractions prepared from isolated rat adipose cells. Forty-eight samples can be read in duplicate within 1 min. Sucrose in concentrations up to 0.7 M interfere only moderately with the assay. A rapid and convenient method is, therefore, now available for multiple protein determinations following sucellular fractionation on sucrose gradients.
' Abbreviations used: BSA, bovine serum albumin; TES, 20 mM Tris, 1 mM EDTA, and 255 mM sucrose.