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Pathogenesis of hodgkin's disease: Separation and culture of different kinds of cells from hodgkin's disease in a sterile isokinetic gradient of ficoll in tissue culture medium

✍ Scribed by Thomas G. Pretlow II; Douglas E. Luberoff; Linda J. Hamilton; Phillip C. Weinberger; William A. Maddox; John R. Durant

Publisher: John Wiley and Sons
Year: 1973
Tongue: English
Weight: 964 KB
Volume: 31
Category: Article
ISSN: 0008-543X
DOI: 10.1002/1097-0142(197305)31:5<1120::aid-cncr2820310513>3.0.co;2-1

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Separation of megakaryocytes from rat bo

Separation of megakaryocytes from rat bone marrow cells using velocity sedimentation in an isokinetic gradient of ficoll in tissue culture medium

✍ Dr. Thomas G. Pretlow; Anthony J. Stinson 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 422 KB

## Abstract A technique for the purification of rat megakaryocytes is described. Velocity sedimentation in a previously described isokinetic gradient of Ficoll (polysucrose) in tissue culture medium was more effective than isopycnic sedimentation for the purification of megakaryocytes and resulted