A simple method for synthesizing [γ-32P]nucleoside triphosphates using [32P]acetylphosphate and acetate kinase

✍ Scribed by Pál I. Bauer; Gyula Várady

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 256 KB
• Volume: 91
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90547-x

✦ Synopsis

A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a final purification step. The entire procedure is performed 8 h or less.