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Preparation of 3′−32P-coenzyme A

✍ Scribed by Eberhard G. Trams; John E. Franklin; Carl J. Lauter

Publisher
John Wiley and Sons
Year
1971
Tongue
French
Weight
138 KB
Volume
7
Category
Article
ISSN
0022-2135

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✦ Synopsis

A convenient labeling technique for the nucleotide portion of CoASH consists of the introduction of 32phosphorus at the 3'-position of the ribosyl moiety of CoA. Adenine pantetheine dinucleotide was prepared according to Wang ( I ) by treatment of CoA with a 3 '-nucleotidase (3.1.3.6). Isotopic phosphorus was incorporated from y-labeled ATP which had been prepared according to Glynn and Chappell by Mr. G. Koval from our laboratory.

EXPERIMENTAL.

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