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A micromethod for the quantitation of cellular proteins in Percoll with the Coomassie brilliant blue dye-binding assay

✍ Scribed by Renaud Vincent; Denis Nadeau

Book ID
107711944
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
584 KB
Volume
135
Category
Article
ISSN
0003-2697

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✍ Hassan Ahmad; M. Saleemuddin πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 785 KB

A sensitive assay procedure for the determination of microgram quantities of immobilized proteins is described. The procedure is based on the property of Coomassie blue G-250 to bind strongly yet reversibly to proteins. The assay involves incubation of the immobilized protein with a solution contain