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Refinement of the Coomassie brilliant blue G assay for quantitative protein determination

✍ Scribed by Bernd-Michael Löffler; Hans Kunze


Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
266 KB
Volume
177
Category
Article
ISSN
0003-2697

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An automatic method for the protein assay using Coomassie Brilliant Blue G-250 was developed and applied to the assay of urinary proteins. In developing the automatic system, the adhesion of protein-bound dye to the walls of the flow cell and tubes was found to be the most troublesome problem, by wh