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An evaluation of the Coomassie brilliant blue G-250 dye-binding method for quantitative protein determination

✍ Scribed by John Pierce; C.H. Suelter


Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
147 KB
Volume
81
Category
Article
ISSN
0003-2697

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Refinement of the Coomassie blue method
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The Coomassie brilliant blue Cl assay for proteins described by Bradford (1976) (Ana!. Biochem. 72, 248) was reexamined. It was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 0.8 to 10 &ml of solution. This unchangi