An automatic method for the protein assay using Coomassie Brilliant Blue G-250 was developed and applied to the assay of urinary proteins. In developing the automatic system, the adhesion of protein-bound dye to the walls of the flow cell and tubes was found to be the most troublesome problem, by wh
β¦ LIBER β¦
Determination of Sepharose-bound protein with Coomassie brilliant blue G-250
β Scribed by R.A. Asryants; I.V. Duszenkova; N.K. Nagradova
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 304 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0003-2697
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A new staining procedure for proteins in polyacrylamide gels has been developed. This procedure, utilizing Coomassie brilliant blue G250, is rapid (as quick as 30 min) and simple to perform, requires little or no destaining, and has a sensitivity of a least 1 pg of protein per band. It can be used t