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Quantitative Determination of Protamines by Coomassie Blue G Assay

✍ Scribed by J.K. Chan; J.W. Thompson; T.A. Gill


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
294 KB
Volume
226
Category
Article
ISSN
0003-2697

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An automatic method for the protein assay using Coomassie Brilliant Blue G-250 was developed and applied to the assay of urinary proteins. In developing the automatic system, the adhesion of protein-bound dye to the walls of the flow cell and tubes was found to be the most troublesome problem, by wh