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A Mathematical Model for the Description of the Coomassie Brilliant Blue Protein Assay

✍ Scribed by B.A. Atherton; E.L. Cunningham; A.G. Splittgerber


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
203 KB
Volume
233
Category
Article
ISSN
0003-2697

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A rapid calorimetric method for the assay of proteolytic enzymes based on the binding of Coomassie brilliant blue G-250 to unhydrolyzed protein substrate is described. Considerable assay time is saved since the method does not require the separation of the hydrolyzed products from the undegraded pro